Protocol for Micropropagation of Two Ficus carica Cultivars

Shoot tips of two fig cultivars (Conadria and Black Mission) proliferated in vitro, were cultured on modified MS medium supplemented with 0.5 mg/l 6-benzylaminopurine (BAP). The present investigation aimed to investigate the effect of different medium salt strengths (Double, full, half and quarter) and different sources of carbon (sucrose and fructose) at different concentrations (0.1 and 0.2 mol/l) supplemented in MS medium on shoot multiplication and rooting stage of studied fig cultivars. Obtained results showed that Black Mission cultivar surpassed Conadria cultivar in shoot number while, Conadria gave higher leaves number and shoot length than Black Mission. Full and double MS medium strengths enhanced both of shoots number and shoot length, significantly. Fructose as a carbon source was better than sucrose for multiplication. The best shoot length and shoot number were obtained when fructose was added to the medium at 0.2 mol/l. In rooting stage, MS medium with half strength increased markedly rooting percentage in comparison with other medium strengths. Rooting percentage, root number, root length and plantlet length of fig shoots were better on MS medium contained fructose (0.1M) as a carbon source compared with those on medium contained sucrose.